Lens Culinaris Lectin (LCA/LcH) (10 mg)

$145.00

Use for hemagglutination and cell agglutination studies. Learn more here.

SKU: A05-0104-10mg

Manufacturer: Medicago

Categories: Lectins, Lens culinaris lectin (LCA/LcH)

Product Description

Medicago AB Products Are Available Worldwide. For Research Use Only. Lens culinaris lectin is isolated from lentil seeds by affinity chromatography. It has 2 subunits and a molecular weight of 46 kDa. The lectin gives two major bands in isoelectrofocusing corresponding to the isomers LCA-A and LCA-B. Lens culinaris is not blood group specific. Its carbohydrate specificity is D-mannose and D-glucose. Adding Mn2+ and Ca2+ to the reconstitution buffer will enhance hemagglutination activity. LCA contains a hemagglutinin A and B mixture that agglutinates human red blood cells but that is not group-specific. Lens culinaris agglutinates a 2% suspension of human erythrocytes at a lectin concentration of 6 g/ml in 0.9% NaCl after 2 hrs at 25C. Adding 60 mM methylmannoside gives an inhibition with a titer that is at least 16-fold lower than the control. LCA/LcH is supplied as a white lyophilized powder from a buffer containing 1 mM CaCl2, 1 mM MnCI2 and 1 mM MgCI2. No preservatives are added. For laboratory use only. Use for hemagglutination and cell agglutination studies. Learn more here.

Product Documents & Features

Hemagglutination. Cell agglutination studies.

Sugar specificity: α-D-mannose and α-D-glucose.
Hemagglutinating activity.
High activity.

Appearance: White lyophilized powder or flocculate.
Source: Lentil seeds.
Molecular weight: 46 kDa.
Sugar specificity: α-D-mannose and α-D-glucose.
Activity: Agglutinates human erythrocytes in a 2% blood suspension with lectin concentration ≤ 8 µg/ml in 0.9% NaCl after 2 hrs at 25°C.
Microorganisms: < 100 CFU/g.
Protein content: > 85%, OD280nm (ε 1.6 = 1 mg/ml).
Identity: SDS-PAGE, three bands - the three isolectins.
Shelf life: > Three years when stored at -20°.