Xenometrix
AMES MPF 98/100 AQUA – with S9 and Positive Controls
Ames MPF 98/100 is a miniaturized, ready to use Ames Test kit in a liquid microplate format according to ISO 11350. It includes all reagents necessary to run 5 samples with strains TA98 and TA100: 5 vials each of semisolid TA98, TA100, plus Booster, Growth -, Exposure -, Reversion Indicator Media, Ampicillin, Positive Controls, lyophilized Post-Mitochondrial Supernatant S9 from Phenobarbital/Naphtoflavone induced rat liver. Not included in the kit are plastic ware and S9 Cofactor solutions. The kits are quality controlled and do not need a separate quality control (phenotypes) like the agar plate Assay in petri dishes when used as an inhouse test.
MacroAmes1 – Penta 3 with 5 Strains – Ames Test Kit in Petri Dishes OCED TG471 – Ready to Use
Salmonella strains with specific genetic modifications are used in the Ames Test, they are unable to grow without the amino acid histidine (his- genotype). Only Salmonella strains that have undergone a genetic mutation, i.e. a mutagenic reversion to histidine prototrophy can survive and grow in absence of histidine (his+ genotype). Thus, only in the presence of mutagenic compounds, an increase in back-mutations occur to this his+ genotype. The Salmonella colonies which are growing on agar plates or in liquid media, are called “revertants”. An increased number of revertants in relation to the strain-specific spontaneous mutation rates obtained with the solvent control is a clear indication for a mutagenic test substance. See also OECD TG471. Many mutagenic compounds are only mutagenic upon metabolization. The Salmonella strains which do not have a metabolic system are therefore exposed to a test compound in the presence of microsomal fraction S9 of a rat liver homogenate. Bacteria are exposed to different concentrations of a test sample (11 mg / strain), as well as a positive and a negative control in a medium containing limited quantities of histidine (S. typhimurium) to support approximately two cell divisions. The cultures in each condition (negative control, test samples and positive controls) are poured in 6 well plates. The cells are exposed to the compound in absence and presence of S9. Rat liver post mitochondrial S9 fraction mimics the effect of the chemical after metabolization. Within three days, cells that have undergone a genetic reversion event to amino acid prototrophy will grow and form colonies. The revertant counts will be compared to those grown in the solvent (negative) control wells. A dose-dependent and significant increase in the number of revertant colonies upon exposure to test sample relative to the solvent controls indicates that the sample is mutagenic. A sterility plate for S9 and buffer is used a contamination control. Service analytics under GLP and non GLP conditions is available, please contact info@xenometrix.ch.
MacroAmes1 – 98/100 Ready to use Ames Test Kit in Petri Dishes
2 Sample Kit with TA98 and TA100, incl. PB/NF induced S9 and Positive Controls, Buffers, Agar, 192 Measuring Points. MacroAmes1 is a ready to use, Ames test kit for the use on petri dishes according to OECD TG471. The kit includes strains, media, PB/NF induced rat liver S9, positive controls, ampicillin, booster solution. All components are quality controlled and come with a certificate of analysis.
Ames-Semisolid D. Typhimutium Strains-250 uL TA102
TA102 – S. typhimurium Tester Strain for Ames Test: for the detection of genotoxic chemicals according to OECD 471for the detection of genotoxic impurities according to ICH M7 Guidelinefor testing waste or surface water according to ISO 11350:2012for the screening of chemicals, pharmaceuticals or cosmetics in a miniaturized assay system (AMES MPF)
Ames-Semisolid E. coli Strains – E. coli pKM101
Ames – Semisolid E.coli Strains – E.coli pKM101.
Ames-Semisolid E. coli Strains – E. coli pKM101
Ames – Semisolid E.coli Strains – E.coli pKM101.
Ames-Semisolid E. coli Strains – E. coli UvrA
Ames – Semisolid E.coli Strains – E.coli uvrA.
Ames-Semisolid S. Typhimurium Strains, TA1537
Ames – Semisolid S. typhimurium Strains – TA1537.
Ames-Semisolid S. Typhimurium Strains, TA1535
Ames – Semisolid S. typhimurium Strains – TA1535.
Ames-Semisolid S. Typhimurium Strains, TA100
Ames – Semisolid S. typhimurium Strains – TA100.
Ames – Semisolid S. Typhimurium Strains, TA98
Ames – Semisolid S. typhimurium Strain – TA98.
Ames Rat Liver S9 (Lyophilized), PB/BN Induced, (2 mL)
Ames Rat Liver S9 (Lyophilized), PB/BN Induced.
Ames Rat Liver S9 (Lyophilized), PB/BN Induced (1 mL)
Ames Rat Liver S9 (Lyophilized), PB/BN Induced.
Ames Rat Liver S9-100 / 1537 Booster Solution
Recommended to reduce possible toxic effects of high S9 concentrations in TA100 and TA1537.
Nitroquinoline-N-oxide – Positive Controls for Ames MPF, umuC (20 micrograms)
4-Nitroquinoline-N-oxide – Positive Controls for Ames MPF, umuC.
Nitrofluorene – Positive Controls for Ames MPF, umuC (20 micrograms)
2-Nitrofluorene – Positive Controls for Ames MPF, umuC.
Aminoacridine – Positive Controls for Ames MPF, umuC (1000 micrograms)
9-Aminoacridine – Positive Controls for Ames MPF, umuC.
2-Aminofluorene – Positive Controls for Ames MPF, Ames II, umuC
2-Aminofluorene – Positive Control.
N4-Aminocytidine – Positive Control for Ames MPF, umuC
N4-Aminocytidine – Positive Control.
Aminoanthracene – Positive Controls for Ames MPF, umuC (100 micrograms)
2-Aminoanthracene – Positive Controls for Ames MPF, umuC.
Ames MPF/Ames II Salmonella Reversion Indicator Medium
Ames MPF Salmonella Reversion Indicator Medium.
Ames MPF 10x Exposure Medium for Ames MPF Aqua Test Kit
Ames MPF 10x Exposure Media – Ready to Use Media.
Ames MPF / Ames II Salmonella Exposure Medium
Ames MPF Salmonella Exposure Medium.
Ames MPF E.coli Reversion Indicator Medium
Ames MPF E.coli Reversion Indicator Medium.
Ames – Liquid Typhimurium Strain, TA98
Ames – Liquid S. typhimurium strain – TA98.
XenoScreen XL YAS
For Research Use Only. XenoScreen XL YAS.
