Medicago
USP Anti-FXa and Anti-Flla Assay for LMWH Buffer (pH 7.4) (1 L) (1 pouch)
Medicago AB Products Are Available Worldwide. For Laboratory Use Only. Pre-weighed powder mix in sealed pouch (Store at 20-25C). The contents of 1 pouch dissolved in 1 L of deionized water yields:0.050 M Tris Buffer pH 7.4 @ 25C,0.150 M NaCI.For use as dilution buffer for standards, samples, and blank in USP method for Anti-FXa and Anti-FIIa activity assays for Low Molecular Weight Heparin (LMWH). USP = United States Pharmacopeia.
USP Anti-FXa and Anti-Flla Assay for LMWH Buffer with PEG (pH 7.4) (1 L) (1 pouch)
Medicago AB Products Are Available Worldwide. For Laboratory Use Only. Pre-weighed powder mix in sealed pouch (Store at 20-25C). The contents of 1 pouch dissolved in 1 L of deionized water yields:0.050 M Tris Buffer pH 7.4 @ 25C,0.150 M NaCI,0.1% (w/v) PEG 6000.For use as dilution buffer for AT, FXa, and thrombin in USP method for Anti-FXa and Anti-FIIa activity assays for Low Molecular Weight Heparin (LMWH). USP = United States Pharmacopeia.
USP-EP Anti-FXa and Anti-Flla Assay for UFH Buffer with PEG (pH 8.4) (1 L) (1 pouch)
Medicago AB Products Are Available Worldwide. For Laboratory Use Only. Pre-weighed powder mix in sealed pouch (Store at 20-25C). The contents of 1 pouch dissolved in 1 L of deionized water yields:0.050 M Tris-HCl Buffer,0.175 M NaCl,0.0075 M Edetate Sodium,2.0 g/l BSA, pH 8.4.For use as dilution buffer for standards, samples, AT, FXa, thrombin, and blank in USP and EP methods for Anti-FXa and Anti-FIIa activity assays for Unfractionated Heparin (UFH). USP = United States Pharmacopeia.EP = European Pharmacopoeia.
USP Heparin BSA Buffer without PEG (1 L) (1 pouch)
Medicago AB Products Are Available Worldwide. For Research Use Only. 1 pouch of USP Heparin BSA Buffer without PEG dissolved in 1 L of deionized water yields:0.050 M Tris-HCl Buffer,0.175 M NaCl,0.0075 M Edetate Sodium,2.0 g/l BSA, pH 8.4.
Stop Solution for TMB (50 mL)
Medicago AB Products Are Available Worldwide. For Research Use Only. Stop Solution specifically developed for the EC-Blue Enhanced Substrate (TMB). Supplied in a 50 ml bottle. For laboratory use only.
EC-Blue Enhanced Substrate (TMB) (5L)
Medicago AB Products Are Available Worldwide. For Research Use Only. EC-Blue Enhanced Substrate (TMB) Substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA applications. TMB Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Color development can be stopped by adding 0.5 M H2SO4, which turns the substrate yellow (450 nm). TMB contains a proprietary ready-to-use chromogen substrate mixture. The solution has a straw-yellow color at room temperature and a light greenish tint when stored at 4C. The color returns to normal when warmed to room temperature. For laboratory use only.
EC-Blue Enhanced Substrate (TMB) (250 mL)
Medicago AB Products Are Available Worldwide. For Research Use Only. EC-Blue Enhanced Substrate (TMB) Substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA applications. TMB Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Color development can be stopped by adding 0.5 M H2SO4 (A10-9500-50), which turns the substrate yellow (450 nm). TMB contains a proprietary ready-to-use chromogen substrate mixture. The solution has a straw-yellow color at room temperature and a light greenish tint when stored at 4C. The color returns to normal when warmed to room temperature. For laboratory use only.
EC-Blue Enhanced Substrate (TMB) (1 L)
Medicago AB Products Are Available Worldwide. For Research Use Only. EC-Blue Enhanced Substrate (TMB) Substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA applications. TMB Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Color development can be stopped by adding 0.5 M H2SO4 (A10-9500-50), which turns the substrate yellow (450 nm). TMB contains a proprietary ready-to-use chromogen substrate mixture. The solution has a straw-yellow color at room temperature and a light greenish tint when stored at 4C. The color returns to normal when warmed to room temperature. For laboratory use only.
EC-Blue Enhanced Substrate (TMB) (100 mL)
Medicago AB Products Are Available Worldwide. For Research Use Only. EC-Blue Enhanced Substrate (TMB) Substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA applications. TMB Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Color development can be stopped by adding 0.5 M H2SO4 (A10-9500-50), which turns the substrate yellow (450 nm). TMB contains a proprietary ready-to-use chromogen substrate mixture. The solution has a straw-yellow color at room temperature and a light greenish tint when stored at 4C. The color returns to normal when warmed to room temperature. For laboratory use only.
EC-Blue Enhanced Substrate (TMB) (25 L)
Medicago AB Products Are Available Worldwide. For Research Use Only. EC-Blue Enhanced Substrate (TMB) Substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA applications. TMB Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Color development can be stopped by adding 0.5 M H2SO4 , which turns the substrate yellow (450 nm). TMB contains a proprietary ready-to-use chromogen substrate mixture. The solution has a straw-yellow color at room temperature and a light greenish tint when stored at 4C. The color returns to normal when warmed to room temperature. For laboratory use only.
Protein L Ligand Leakage ELISA XL (1 kit)
Medicago AB Products Are Available Worldwide. For Research Use Only. This Protein L Ligand Leakage ELISA Kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions. It is a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody. In this assay, the samples are boiled to separate the Ig/Ig-fragments from the Protein L in the solution, centrifuged, and incubated in the strip wells. The bound Protein L is then detected by adding a horseradish peroxidase (HRP)-conjugated anti-Protein L IgY antibody. Substrate is added to induce a colour change which is proportional to the amount of bound Protein L from the sample. The colour change is quantified spectrophotometrically at 450 nm by using a plate reader. The amount of protein L in the sample is determined by using a standard curve prepared in a reference sample. The reference sample should have the same concentration of Ig or Ig-fragments and preferably the same buffer composition as the elution samples from the Protein L affinity chromatography medium.
However, it should be prepared using another purification strategy, e.g. ion exchange chromatography. Once a reference sample is created it can be aliquoted and used for several different protein L ELISA assays.
Protein L Ligand Leakage ELISA (Standard Kit)
Medicago AB Products Are Available Worldwide. For Research Use Only. This Protein L Ligand Leakage ELISA Kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions. It is a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody. In this assay, the samples are boiled to separate the Ig/Ig-fragments from the Protein L in the solution, centrifuged, and incubated in the strip wells. The bound Protein L is then detected by adding a horseradish peroxidase (HRP)-conjugated anti-Protein L IgY antibody. Substrate is added to induce a colour change which is proportional to the amount of bound Protein L from the sample. The colour change is quantified spectrophotometrically at 450 nm by using a plate reader. The amount of protein L in the sample is determined by using a standard curve prepared in a reference sample. The reference sample should have the same concentration of Ig or Ig-fragments and preferably the same buffer composition as the elution samples from the Protein L affinity chromatography medium. However, it should be prepared using another purification strategy, e.g. ion exchange chromatography. Once a reference sample is created it can be aliquoted and used for several different protein L ELISA assays.
Phosphate Buffered Saline (PBS) without Potassium (pH 7.4) (100 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Medicago’s Phosphate Buffered Saline (PBS) without potassium is specifically developed for biochemical processing and immunological /microbiological laboratories. It is supplied in two formulations (low and high buffer strength) as pre-weighed tablets in bottles or convenient blister packs.
Phosphate Buffered Saline (PBS) (pH 7.2) (1 L) (100 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. One of the most commonly used biological buffers. Isotonic and non-toxic to cells. Medicago’s PBS is specifically developed for immunological and microbiological laboratories. It is provided in sealed pouches and as pre-weighed tablets in bottles or convenient blister packs. Thirteen standard-sized packages and volumes range from 100 ml to 100 litres.
PBS tablets pH 7.2 (10 tablets x 1000 mL/tablet)
Medicago AB Products are Available Worldwide. For Research Use Only. Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns (1). References: 1. A peptide carrier for the delivery of biologically active proteins into mammalian cells. (2001) MC Morris, J Depollier, J Mery, F Heitz.Nature Biotechnology 19, 1173 – 1176.
Phosphate Buffered Saline (PBS) without Potassium (1 L) (100 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Medicago’s Phosphate Buffered Saline (PBS) without potassium is specifically developed for biochemical processing and immunological /microbiological laboratories. It is supplied in two formulations (low and high buffer strength) as pre-weighed tablets in bottles or convenient blister packs.
PBS without potassium pH 7.4 (10 tablets x 1000 mL/tablet)
Medicago AB Products are Available Worldwide. For Research Use Only. Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns (1). References: 1. A peptide carrier for the delivery of biologically active proteins into mammalian cells. (2001) MC Morris, J Depollier, J Mery, F Heitz.Nature Biotechnology 19, 1173 – 1176.
Phosphate Buffered Saline with Tween 20 (PBS-T) (pH 7.4) (1 L) (100 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Phosphate Buffered Saline with Tween 20 (PBS-T) buffer contains the non-ionic detergent Tween 20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. Decreasing the non-specific binding and staining makes ELISA results and blots easier to interpret.
Phosphate Buffered Saline with Tween 20 (PBS-T) (pH 7.4) (10 tablets x 1000 mL/bottle)
Medicago AB Products Are Available Worldwide. For Research Use Only. Phosphate Buffered Saline with Tween 20 (PBS-T) buffer contains the non-ionic detergent Tween 20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. Decreasing the non-specific binding and staining makes ELISA results and blots easier to interpret.
Phosphate Buffered Saline (PBS) (pH 7.4) (1 L) (100 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. One of the most commonly used biological buffers. Isotonic and non-toxic to cells. Medicago’s PBS is specifically developed for immunological and microbiological laboratories. It is provided in sealed pouches and as pre-weighed tablets in bottles or convenient blister packs. Thirteen standard-sized packages and volumes range from 100 mL to 100 L. References: 1. A peptide carrier for the delivery of biologically active proteins into mammalian cells. (2001) MC Morris, J Depollier, J Mery, F Heitz. Nature Biotechnology 19, 1173 – 1176.
PBS tablets pH 7.4 (10 tablets x 1000 mL/tablet)
Medicago AB Products are Available Worldwide. For Research Use Only. Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns (1). References: 1. A peptide carrier for the delivery of biologically active proteins into mammalian cells. (2001) MC Morris, J Depollier, J Mery, F Heitz.Nature Biotechnology 19, 1173 – 1176.
Sodium Chloride (NaCl) (1 L) (100 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Sodium Chloride is usually used in laboratory routines. The product is supplied in convenient exactly pre-weighed tablets and as powder in pouches.
Sodium Chloride (NaCl) (1 L) (10 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Sodium Chloride is usually used in laboratory routines. The product is supplied in convenient exactly pre-weighed tablets and as powder in pouches.
Sodium Chloride (NaCl) (100 mL) (100 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Sodium Chloride is usually used in laboratory routines. The product is supplied in convenient exactly pre-weighed tablets and as powder in pouches.
SSPE Tablets (200 mL) (100 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Medicago’s SSPE buffer is specifically formulated for hybridization in Northern and Southern blotting and for wash steps in microarray techniques. The buffer can be used in various applications within molecular biology such as immunocyto-chemistry and cell culture procedures. It is suitable as wash buffer for some cell cultures and for resuspending cells for FACS (fluorescence-activated cell sorter) techniques, in order to attain good cell sorting.
SSPE Tablets (200 mL) (100 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Medicago’s SSPE buffer is specifically formulated for hybridization in Northern and Southern blotting and for wash steps in microarray techniques. The buffer can be used in various applications within molecular biology such as immunocyto-chemistry and cell culture procedures. It is suitable as wash buffer for some cell cultures and for resuspending cells for FACS (fluorescence-activated cell sorter) techniques, in order to attain good cell sorting.
Carbonate-Bicarbonate Buffer with Azide (pH 9.6) (100 mL) (8 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Specifically developed for protein-coating procedures on microtiter plates and plastic for RIA and EIA
techniques. The buffer is supplied in tablets with two formulations, one of which contains 0.05% sodium azide as preservative.
Carbonate-Bicarbonate Buffer with Azide (pH 9.6) (100 mL) (8 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Specifically developed for protein-coating procedures on microtiter plates and plastic for RIA and EIA
techniques. The buffer is supplied in tablets with two formulations, one of which contains 0.05% sodium azide as preservative.
Carbonate-Bicarbonate Buffer with Azide (pH 9.6) (100 mL) (50 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Specifically developed for protein-coating procedures on microtiter plates and plastic for RIA and EIA
techniques. The buffer is supplied in tablets with two formulations, one of which contains 0.05% sodium azide as preservative.
Carbonate-Bicarbonate Buffer (pH 9.6) (100 mL) (24 tablets)
Medicago AB Products Are Available Worldwide. For Research Use Only. Specifically developed for protein-coating procedures on microtiter plates and plastic for RIA and EIA techniques. The buffer is supplied in tablets with two formulations, one of which contains 0.05% sodium azide as preservative.
