EC-Blue Enhanced Substrate (TMB) (1 L)
$415.00
Product Description
Medicago AB Products Are Available Worldwide. For Research Use Only. EC-Blue Enhanced Substrate (TMB) Substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA applications. TMB Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Color development can be stopped by adding 0.5 M H2SO4 (A10-9500-50), which turns the substrate yellow (450 nm). TMB contains a proprietary ready-to-use chromogen substrate mixture. The solution has a straw-yellow color at room temperature and a light greenish tint when stored at 4C. The color returns to normal when warmed to room temperature. For laboratory use only.
1L. For Research Use Only. Recommended for ELISA procedures. See more details here.
Product Documents & Features
- Ready-to-use liquid substrate.
- Substrate for assays using horseradish peroxidase (HRP).
- Low background.
- High sensitivity.
- Long stability.
- Appearance: Straw yellow color at room temperature. Slightly green tint when stored at +4°C. Color returns to yellow when stored at room temperature.
- Protein content: One bottle of EC Blue consist of a solution containing chromogen substrate reagents already mixed, pH 3, 8.
- Functional characteristics: Functional testing shows less than 10% average standard deviation from previously approved lots of chromogen. Test performed in the same assay. Background optical densities shows less than 10% deviation from that of previously approved lots when tested in the same assay.
- Storage: 2°C to 8°C.
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